Monitoring of  drinking  water quality in Baghdad city  by using polymerase chain reaction(PCR)

	Monitoring of drinking water quality in Baghdad city by using polymerase chain reaction(PCR)
Journal of University of Babylon
Article 1, Volume 22, Issue 3, 2014, Pages 1087-1093
Abstract
The monitoring of water Iraq quality with regard to detection of microbial contamination, mainly depend on time consuming conventional culture methods. Therefore to ensure continuous good water quality supply, sensitive and good detection methods are important to be available always, specifically polymerase chain reaction (PCR), for direct quick, sensitive and accurate detection for the presence of microbial pathogens in drinking water directly without the need for culturing and isolating the pathogens. 300 water sample were taken from different regions in Baghdad city (270)samples were positive for the fecal and total coliform with routine diagnosis methods, in the same time (200) sample were positive for E.coli The PCR amplification assay detected the presence of bacteria in (250) out of 300 water samples depending on the Uid, LacZ genes. Twenty(20) out (300) water samples showed a positive result for the MF and negative result for PCR using primer pair specific for the genus TC and E.coli .

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