Study of some virulence factors of some urepathogenic bacteria

Ahmed AL-Hamawandi, Jwan

	Study of some virulence factors of some urepathogenic bacteria
Journal of University of Babylon
Article 1, Volume 22, Issue 4, 2014, Pages 1355-1362
Author
Jwan Ahmed AL-Hamawandi
Abstract
In this study, sixty urine samples (60 samples) were obtained from patients suffering from urinary tract infections (UTIs), who have attending to Hilla Teaching Hospital for Surgery Department of Urology for the period from April to August 2012. These samples were identification by biochemical test to diagnosed types of bacteria, it was found that 20(33.3%) of the isolates were E. coli while other gram negatives were found in small in number, 2(3.3%) isolates for Pseudomonas aeruginosa, 7(11.6%) isolates for Klebseilla pneumonia and one(1.6%) isolates for Proteus mirabilis. On the other hand the isolated Gram positive bacteria were 18(30%) for Staphylococcus aureus and 5 (8.3%) Staphylococcus fecalis, 3(5%) isolates for Streptococcus agallagtia and 4(6.6%) isolates from streptococcus pyogenes. Mannose resistance hemoglutinin type IV was studied, it was found that all isolated have this variance factor. Also, hemolysin production by gram- positive and gram-negative bacteria isolated from urinary tract infection was studied, and it was found that E. coli, Pseudomonas, and Staphylococcus aureus, Staphylococcus fecalis, streptococcus pyogenes. Proteus mirabilis have able to produce hemolysin as show narrow zone of β- hemolysis around colonies on blood agar plate, while Klebseilla, were non hemolytic which no color change around the bacterial colonies on blood agar. Extracellular protease production by gram- positive and gram-negative bacteria isolated from urinary tract infection was detected in this study, and it was found that all isolates of this study (E. coli, Pseudomonas, Staphylococcus aureus, Staphylococcus fecalis streptococcus pyogenes, Proteus mirabilis and Klebseilla) have able to produce protease production in M9 media (supplemented with 20% glucose and 1% gelatin) after 24 hours of incubation. A clear hallo of transparent area was found around the colony after the addition of 3 ml of 5% trichloroacetic acid. On the other hand, it was detection of lipase production, it was found that Klebseilla and Proteus mirabilis give appositive results, while E. coli, Pseudomonas, Staphylococcus aureus, Staphylococcus fecalis, Streptococcus pyogenes give variable results.

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