Screening Of Cellulase Activity Produced From Pseudomonas Fluorescens

Mohammed Jarallah, Eman; Ra, Ali; Abdul-kadhim, Ad

	Screening Of Cellulase Activity Produced From Pseudomonas Fluorescens
Journal of University of Babylon
Article 1, Volume 21, Issue 3, 2013, Pages 849-854
Authors
Eman mohammed jarallah; Ali Ra; ad Abdul-kadhim
Abstract
Eighteen cellulytic bacterial isolates were isolated from local agriculture soils from different area in Hilla city during 5 Months (October 2010 to march 2011).These isolates were purified using subculture method on blood agar for many times to get single colonies. All these bacterial isolates identified depending on cultural and biochemical characteristics. The results showed that all bacterial isolates belonging to Pseudomonas fluoresens includes four biovars of this species which were biovar 1, biovar 2, biovar 3and biovar 4 . Cellulytic activity was screened according to a clear zone diameter (mm ) that formed around bacterial growth on carboxymethylcellulose (CMC ) agar which formed when adding a lougl's iodine solution, The most efficient isolate was P.fluoresens biovar 4 which give 23 mm as clear zone , while the less efficient one was P.fluoresens biovar 1which give 10 mm as clear zone . The crude enzymes that produced by a given isolates were precipitate by ammonium sulfate ,Diaylsis and the enzyme activity was determined . One unit of CMCase activity was expressed as 1μmol of glucose librated per ml enzyme per minute. The four species biovars isolated in this study give enzyme activity reach to 1.717 , 1.431 , 2.146 , 4.574 u/ ml for biovars 1,2,3and 4 , respectively .

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