Effect of Hot Aqueous Extract of the Fungus Agaricus bisporus on Cancer and Normal Cell Lines In vitro.

H. Hamzaa, Israa

	Effect of Hot Aqueous Extract of the Fungus Agaricus bisporus on Cancer and Normal Cell Lines In vitro.
Al-Mustansiriyah Journal of Science
Article 1, Volume 22, Issue 4, 2011, Pages 79-93
Author
Israa H. Hamzaa
Abstract
This study was designed to investigate the inhibition effect of the hot aqueous extract of Agaricus bisporus on cancer cell lines and normal cells in vitro. The examination included using two types of cancer cell lines cultured in vitro which were (murine mammary adenocarcinoma AMN-3 cell line, human rhabdomyosarcoma RD cell line) and comparing them with the transformed normal cell line from the Rat embryo fibroblasts Ref cell line by using tissue culture technology in vitro. Ten different concentrations starting from 87.5 to 0.17 mg/ml of two fold dilution for extract were prepared and tested on each cell line, with three replicates for each concentration. Periods of exposure of cell lines were measured at 24, 48 and 72-hours intervals in a microtitration plate under complete sterile conditions. The study of inhibition test in tissue culture showed that hot crude aqueous extract had inhibition effect depending on the type of the cells, amount of the dose and the time of exposure , the highest effect was noticed at the high concentrations of extract after 48 and 72 hours of exposure for each of the two cancer cell lines. The hot aqueous extract showed an inhibitory effect on the growth of AMN3 in all concentrations at the exposure period of 48 and 72 hours and in high concentrations at the period of 24 hours, also it showed an inhibition effect in the RD cancer cell line at high concentrations only, it did not have any inhibitory effect in the REF cell line, that means it does not cause any negative effect in the normal cells despite having an inhibitory effect against cancer cells.

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