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A Study on Relationship of Small Plasmid with Multidrug-Resistance Pattern in Pathogenic E.coli Local Isolates. | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 22, Issue 4, 2011, Pages 94-101 | ||
| Author | ||
| Zainab M. Naji | ||
| Abstract | ||
| Eighty clinical isolates of Escherichia coli isolated from UTI patient at Medical City Hospital were studied to investigate antimicrobial resistance to nine antimicrobial agents. The majority of multiple drug resistance isolates showed different antibiotics resistance by using disc diffusion method. The current isolates were resistance to Cloxacillin and Trimethprim 100%, Amoxicillin 90% while the Gentamycine and Ciprofloxine 60%. Afterwards, three isolates no.2, no.4, no.7, were subjected to plasmid isolation using modifying alkaline methods without Lysozyme and phenol chlorophorm. Isolates were carrying small plasmid estimated about (2-4) kb cured with Acridin orange , the mutated isolates loss their plasmid and antibiotic resistance about 80% to Amoxicillin Gentamycine, Cloxacillin, and Cephtaxim in isolate no.2 and about 50% to Gentamycine and amoxicillin in isolates no.4 and no.7. | ||
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