Study of Some Changes in Spleen among Golden Hamsters Vaccinated with Killed Leishmania tropica

Ja, Eed; Fer,; Sa, Harith

	Study of Some Changes in Spleen among Golden Hamsters Vaccinated with Killed Leishmania tropica
Al-Mustansiriyah Journal of Science
Article 1, Volume 22, Issue 6, 2011, Pages 287-298
Authors
eed Ja; ; Harith Sa
Abstract
Different factors related with spleen (histopathological changes , spleen weight ratio , length , number of parasite , impression and culture of spleen) were screened in four groups of golden hamsters, inoculated with one , two and three doses of different antigens as following : Group 1 inoculated with autoclaved killed Leishmania tropica , Group 2 inoculated with BCG vaccine alone while Group 3 Inoculated with mixed vaccines (autoclaved killed Leishmania + BCG) and Group 4 inoculated with phosphate buffer saline which considerd as control group , in order to evaluate the efficacy of the previous antigen against challenge infection with virulent Leishmania tropica isolate along five times follow up (30, 45, 60, 75, and 90) days after infection. Group 3 which inoculated with one , two and three doses of mixed vaccine, was considered as the best vaccine in this study because animal inoculated with this vaccine showed the following results compared with other antigens: - Low or moderate histopathological changes. - Less significant spleen weight ratio (1.36±0.1) mg/gm , (1.24 ±0.08) and mg/gm (1.21±0.05) mg/gm respectively. - Less significant spleen length (24.4±0.07) mm , (23.7±0.6) mm and (23.4±0.1) mm respectively - Less but no significant amastigote count (1.4 ±0.01) , (1.32±0.05) and (1.42±0.03) million parasites respectively - Negative spleen culture along 90 days of following up.

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