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Production of Pyrethrins in Tissue Cultures of Pyrethrum (Chrysanthemum cinerariaefolium( | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 22, Issue 6, 2011, Pages 307-316 | ||
| Author | ||
| Salam A. ahmed | ||
| Abstract | ||
| This study was carried out in an attempt to increase the production of pyrethrins in tissue cultures of pyrethrum (Chrysanthemum cinerariaefolium) on Murashige and Skoog's (MS) medium supplemented with different concentrations of plant growth regulators. Benzyl adenine (BA) and 2,4-dichlorophenoxy acetic acid (2,4-D) were tested for their potential in callus induction. Callus was induced on leaf explants using MS medium supplemented with 1 mg/l BA and 5 mg/l 2,4-D and on petal explants using 1 mg/l BA and 10 mg/l 2,4-D. those combinations were used for callus initiation and maintenance. Extracts of plant leaves, petals and callus tissues were prepared and investigated for pyrethrin content. Extracts contained flavonoids, terpenes, tannins, resins, essential oils and steroids. HPLC analysis was performed for detection of pyrethrins in the extracts. Results showed that pyrethrin concentration in callus culture extracts was higher than that of intact plant extracts. The concentration of pyrethrin significantly increased on a medium supplemented with 5 mg/l BA and 10 mg/l 2,4-D for leaf explants and 5 mg/l BA and 5 mg/l 2,4-D for petal explants. | ||
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