Detection of Neisseria meningitidis in blood samples by PCR technique and bacterial Culture

	Detection of Neisseria meningitidis in blood samples by PCR technique and bacterial Culture
journal of kerbala university
Article 1, Volume 9, Issue 4, 2013, Pages 125-133 PDF (0 K)
Abstract
The Meningitis of important disease and controversial attention as it gives some conditions as a result of culture negative in spite of the presence of symptoms and clinical inflammatory response was due to the treatment pre- antibiotic , so it was chosen this study to shed light on identifying diagnostic accuracy and clinical benefit of the methods used to detect for Neisseria meningitidis bacteria in samples of blood . Included the current study, 50 cases of suspicious infected with meningitis or septicemia from both sex and by age from 40 day to 14 year outpatient hospital Karbala education for children and Hussein Hospital of education and who have been diagnosed clinically by specialist doctors at the hospital since collected samples of blood were withdrawn for the same people under study and admitted to the emergency unit or intensive care unit (ICU) or transitional diseases unit. blood samples were cultured in traditional media, as well as the DNA was extracted from bacterial samples of blood for the purpose of detecting the presence of the ctrA gene using a technique the PCR. The study results showed that the routine culture method gave the bacteriological test result negative rate of 0.0% for blood samples while when using the PCR technique was the number of cases diagnosed to the presence of bacterial gene 15 cases ( 30.0 % ) of the total blood samples . well as the results showed high incidence of bacterial meningitis disease caused by N. meningitidis among males than females , with total cases of the presence in the blood sample of male patients by the way of PCR were nine cases ( 16.1% ) and females six cases ( 13.6% ), In light of the presented results of the study conclude that the PCR technique is the way to the most useful in the detection of bacteria in blood samples of patients with meningitis cases questionable.

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