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The Effects of Magnetic Fields on Some Biological Activities of Pseudomonas Aeruginosa | ||
| Diyala Journal of Medicine | ||
| Article 1, Volume 5, Issue 1, 2013, Pages 29-35 | ||
| Authors | ||
| Fouad Houssein Kamel; Chiman Hameed Saeed; Saleem Saaed Qader | ||
| Abstract | ||
| Background: A magnetic field is the area of influence exerted by a magnetic force. This field is normally focused along two poles. Most magnetic objects are composed of many small fields called domains. A wide variety of methods have been reported in the literatures which are directed to the use of magnetic energy as a diagnostic technique and also for the treatment of diseases in humans and animals. Aims: To investigate the effects of different levels of static magnetic field on the ultra structure of Pseudomonas aeruginosa bacterium as well as their colony morphological changes. Materials and Method: Locally prepared dipolar static magnetic field of strength 400, 800, 1200 and 1600 Gauss were used in this study measured by Teslameter. Pseudomonas aeruginosa isolated from ten urinary tract infected patients. The samples collected from Rizgary hospital in Erbil during period from January to June 2013, then identified by using API (Analytical Profile Index) 20 E test systems in Hawler Medical Research Center. Equal volumes of broth nutrient culture media of bacteria were exposed to the magnetic field for 24 hour. Furthermore, the bacterial growth subculture tested for morphological and biological activity after API 20 E test of treated Pseudomonas aeruginosa culture media compared with untreated negative control samples. Results: Results indicated that exposure of the microorganisms to demonstrated magnetic field caused pronounced changes in biological activity of enzymes TDA( Tryptophane deaminase), GLU( Glucose fermentation/oxidation), ARA(Arabinose fermentation/oxidation) were observed on the cell growth. On the other hand, changes in morphology of the Pseudomonas aeruginosa colonies were observed on MacConkey agar and became smaller in size with elastic phenomena. Conclusions: We concluded that the magnetic field could change bacterial biological activity on sugar fermentation and colony morphology of bacterial due to mutation. | ||
| Keywords | ||
| KEYWORDS; optical density; pseudomonas aeruginosa; mutation | ||
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