Isolation of Campylobacter bacteria that causing diarrhea in patients of  Tikrit city and diagnose it by polymerase chain reaction PCR

Khudhair Abbas Al-douri, Wahran; Osama Nadhom Nijris, Dr.

	Isolation of Campylobacter bacteria that causing diarrhea in patients of Tikrit city and diagnose it by polymerase chain reaction PCR
Medical Journal of Tikrit
Article 1, Volume 19, Issue 2, 2013, Pages 260-279
Authors
Wahran Khudhair Abbas Al-douri; Dr. Osama Nadhom Nijris
Abstract
This study has been included collection of one hundred fifty 150 stool samples which were 120 watery and 30 once bloody, collected from children that affected by diarrhea there age range is 1-5 years, who admitted to Tikrit teaching hospital, at time from September 2012 till March 2013. Campylobacter isolated from 5 samples by using classical methods of diagnosis which include phenotypic tests represented by studying culturing feature of growing groups by noting the shape, color and size of these groups, in addition to studying of microscopic features of these groups throughout preparation of gram stain and doing of biochemical and physiological tests as oxidase, catalase, urease, hippurate hydrolysis test, discovery of H2S, their ability to grow in 27, 37, 42 centigrade degree of temperature, nitrate reduction test, their ability to tolerate salt, growing on macConky solid media, motility test and sensitivity to the antibiotics as naldixic acid and cephalothin. All these tests reveals that's all the causative agents of diarrhea belonging to species of C.jejuni. Isolation was been achieved by two methods; the first one by use of selective media (Campylobacter agar), and the second by use of Millipore filters of 0.45 μm diameter, this percentage represents just 3.3% of total number of samples, divided as 3 watery stool samples and 2 of bloody stool samples. Comparison was made between results of classical diagnosis methods and advanced methods of diagnosis by use of PCR, PCR diagnosis methods did by using primers that’s antagonize the DNA chain which carry Vac gene, the gene responsible for production of C. jejuni special toxin (Vacuolating cytotoxin), and SADC gene that’s functioning as serine amino acid transporter protein producer. The results obtained by use of PCR are showing presence of Vac and SADC genes in 4 samples only, while the 5th sample did not give any result, which was one of watery stool samples. For that the diagnosis by PCR more accurate than the diagnosis by classical methods.

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