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DETERMINATION OF THE OPTIMUM CONDITIONS FOR THE PRODUCTION OF AGARASE FROM LOCAL ISOLATE BACILLUS SP. H12 | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 1, 2010, Pages 56-70 | ||
| Abstract | ||
| Twenty seven bacterial local isolates of Bacillus sp. were obtained from soil samples. The isolates were tested for agarase production on solid media; fourteen isolates were able to develop clear zone around the bacterial growth after floating the plates with iodine reagent (Lugol´s solution), with clear zone diameter between (1.08-1.11cm). There were further treated in submerged culture which led to selection of Bacillus sp. H12, since it was the most efficient that gave higher specific activity of agarase 11.73 unit/mg protein. The optimum conditions for agarase production using submerged cultures were 0.3% agarose as carbon source, 0.5% beef extract and peptone as nitrogen sources, 0.5% sodium chloride, at initial pH 8 and inoculum size 1×108cell/ml at 37˚C for 24 hrs using shaker incubator at 150 rpm. | ||
| Keywords | ||
| Agarase production; Bacillus sp | ||
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