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MUTAGENECITY EFFECT AND ANTIMUTAGENECITY OF ALCOHOLIC EXTRACT OF SALVIA OFFICINALIS USING BACTERIAL SYSTEM | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 1, 2010, Pages 71-81 | ||
| Abstract | ||
| This study was conducted to detect the toxic and mutagenic and antimutagenic effects of the alcoholic plant extract Salvia officinalis. The optimal concentration of alcoholic extract was 150micrograms/ml, and compared its effectiveness against Methotrexate (MTX) as a mutagenic chemical, and ultraviolet radiation (UV) as a mutagen and using physical test system (in vitro) individually for each nested transactions of the extract with MTX and UV before, within, and after treatment using bacterial system (G-system) depending on plant's survival frection (Sx) to study the effects of mutation and induced resistance to the antibiotic streptomycin and rifampicin as genetic markers. The results showed the effect of interference between the optimal concentration of the extract and mutagen on the survival factor (Sx), increasing of the values of the survival factor of the system isolates to normal values compared to positive control (MTX or UV only), and also showed the results of the overlap between the optimal concentration of the extract and treatment with MTX in induction resistance mutations for streptomycin and rifampicin. MTX had no effect in induction resistance to two anti-biotics before, within, and after treatment with MTX for the three isolates and thus the alcoholic extract was suppress or repair mutations and provided 100% protection of bacterial cells. While the results of the effect of interference between the optimal concentration of the extract and UV treatment showed no any effect in induction resistance to rifampicin for all treatment and all the three isolates. The same as streptomycin, this indicate that treatment of bacterial isolates with plant alcoholic extract with and after exposure to altraridet radiation, while the percentage of mutations repair and protection before UV exposure was 93.6 - 98.3% . | ||
| Keywords | ||
| Salvia officinalis; Sage; alcoholic extract; flavonoides | ||
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