- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
MOLECULAR GENETIC STUDY ON BETA-THALASSEMIA MAJOR IN SOME IRAQI PATIENTS | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 1, 2010, Pages 115-123 | ||
| Abstract | ||
| The objective of this project was to establish the molecular genetics of β-thalassemi in the Iraq. Genomic DNA was isolated from the blood of 75 patients who were diagnosed clinically and hematologically as β-thalassemia major and from 20 blood samples of apparently healthy individuals as control, all these samples was collected from Baghdad thalassemic center at Ibn-Al-Baladi pediatrics hospital. Polymerase chain reaction (PCR) was used for amplification of 4 regions in β-globin gene. The data show that IVS-1 nt 110 mutations are the most common in the population. Codon 39 mutation was also found with relatively high incidence. One of the subjects had a compound mutation which included IVS-1 nt 110 and CD8 mutations. Eighteen of the β-thalassemia major patients showed none of the four common mutations. | ||
| Keywords | ||
| thalassemi; IVS; mutations; Codon; Codon CD | ||
|
Statistics Article View: 195 PDF Download: 49 |
||