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PRODUCTION OF POLYMYXIN ANTIBIOTIC FROM LOCALLY ISOLATEDPAENIBACILLUS PLYMAYXA A3 | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 8, Issue 1, 2009, Pages 289-303 | ||
| Abstract | ||
| Twenty five soil sample from different locations of Baghdad city were collected during February, March and April 2004. Eighty five isolates of the genus Bacillus were obtainted. The ability of these isolates to produce antibiotics against the test organism Pseudomonas aeruginosa was tested in solid media; Six isolates of them were antibiotic producers. After characterization four isolates were found to be Paenibacillus polymyxa, and these isolates showed better production of the polymyxin and one of them was the best and designated as P. polymyxa A3. The optimum cultural conditions for polymyxin production by P. polymyxa A3 were studied. Best results was obtained when the isolate P. polymyxa A3 was grown on media composed of 0.2% glucose, 0.15% ammonium chloride, 0.25% dipotassium hydrogen orthophosphat and 0.25% Sodium chloride, with initial pH of 7 and after 24 hrs of incubation at 37°C. | ||
| Keywords | ||
| Polymyxin; Paenibacillus Polymayxa A; Production | ||
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