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PRODUCTION OF MICROBIAL RENNET ENZYME FROM RHIZOMUCOR MIEHEI IN SOLID STATE FERMENTATION | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 8, Issue 1, 2009, Pages 314-325 | ||
| Abstract | ||
| Ten fungal isolate were selected which include Mucor, Rhizopus from different habitat.Tests conducted for the isolation of the best isolates that produce rennet.Results showed that isolate MucorT4 were the best for identification. The husks rice media was best productive media in solid state fermentation and the optimal condition for production use moisturizing ratio 1:1.6(milliliter distilled water from gram media), pH=6, temperature 40ºC, quantity of inoculums volume 1ml limits 1000000 spore/gm media and incubate for 3 days. It also extracted the enzyme from the production media using phosphate regulator concentration of 0.2 molar pH=6. The estimated effectiveness agglutination for the producer isolation which recorded the highest rate possible within the standards as determined through suitability of milk agglutination and an alternative to traditional rennet. | ||
| Keywords | ||
| Rennet; Rhizomucor miehei; SolidState Fermentation | ||
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