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USING UREASE ENZYME PRODUCED FROM PROTEUS MIRABILISIN THE ESTIMATION OF BLOOD UREA CONCENTRATION | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 8, Issue 1, 2009, Pages 367-374 | ||
| Abstract | ||
| This study concerned the usage of purified urase enzyme which purified from Proteus mirabilis local isolates that was isolated from UTI patients. This enzyme is extracted and purified by ammonium sulphate precipitation method with saturation percentage of 50% and 245 purification fold and enzyme yield of 24% in the estimation of the blood urea concentrationBrothelote method in both kits. The local kit in which the urase enzyme was the principal constituent and the trade kit that supplied by Biocon Company. A comparison was made between the results of urea concentration obtained using both, local kit and standard Biocon kit using statistical analysis T-Test. It was found that there is no significant differences (at confidence level 95%) between the results obtained from the two kits. | ||
| Keywords | ||
| Urase enzyme; Proteus mirabilis | ||
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