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EFFICACY EVALUATION OF INACTIVATED LOCAL VACCINE AGAINST NEWCASTLE DISEASE IN BROILERS | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 8, Issue 1, 2009, Pages 412-418 | ||
| Abstract | ||
| Locally prepared vaccine, precipitated with alum and sodium hydroxide (IAPSHV) in embryonated chicken eggs. Different programs were carried out to evaluate its efficacy. Two hundred and fifty broiler chicks were divided equally into five groups. Different vaccination programs were used a follow. G1: vaccinated with live attenuated vaccine (B1) by intraocular route at 1, 10, 17 and 27 days of age. G2: vaccinated with live attenuated vaccine (B1) by intraocular route at 1 day of age and vaccinated also with (IAPSHV) at 1 day of age. G4: Vaccinated with (IAPSHV) only, by subcutaneous route at 1 day of age. G5: Control group left with out any vaccine. Indirect (ELISA) and Haemagglutination inhibition tests (HI) were used for evaluation of antibodies titer before and after vaccination, challenge test also used with virulent strain of Newcastle disease virus. Results showed that G3 gave the highest titer by both ELISA and (HI) test and low mortality in challenge test. It was concluded that (IAPSHV) at 7 day of age with attenuated live vaccine at the same age gave the best result than other groups. The applied program, and (IAPSHA) vaccine is recommended. | ||
| Keywords | ||
| Newcastle Disease; Inactivated vaccine; Broilers | ||
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