EFFECT OF POLLUTION ON REPRODUCTION TRAITS AND CYTOGENETICS OF SHEEP IN AL-TUWAITHA REGION, BAGHDAD

	EFFECT OF POLLUTION ON REPRODUCTION TRAITS AND CYTOGENETICS OF SHEEP IN AL-TUWAITHA REGION, BAGHDAD
Iraqi Journal of Biotechnology
Article 1, Volume 8, Issue 2, 2009, Pages 521-531
Abstract
The aim of study was to record the effect of radiation and pollution on Iraqi sheep fertility, semen physical characteristics and lymphocyte cell chromosomes changes. Blood was collected from sixty sheep, of them 30 grazing in Al-Tuwaitha area, for lymphocytes cultivation, cytogenetics to do analysis and chromosomal examination by using BudR for DNA molecule. The results indicated that there was chromosomal changes in the animals were exposed to the pollution represented in reduce in Blasto index 22.75±0.5, mitotic index 0.954±0.07 and cell cycle progression, while there was a high significant in sister chromatid exchange(SCE) 7.81±0.30. Semen were collected from ten rams divided into two equal groups, control group were obtained from Veterinary Medicine and Al-Shuala, the second group were obtained from Al-Tuwaitha in Baghdad. The results showed significant differences at P<0.05 level in dead and abnormal sperms percentages between two groups. Also, there was a decrease in fertility and number of birth in 24 ewes which was naturally inseminated from rams grazing in Al-Tuwaitha area, comparing with 24 ewes inseminated from normal rams. The study concluded chromosomal changes in chromatied exchanges with decrease in lymphocyte division. The testes functions was effected when abnormal chromosomes sperms appeared in semen which indicated the genetics changes in sperm chromosomes.
Keywords
Pollution; cytogenetics; sheep; Chromatid; lymphocyte; Sperm abnormalities

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