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PHSIO-CHEMICAL AND SPECTRAL CHARACTERIZATIOIN | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 8, Issue 2, 2009, Pages 532-540 | ||
| Abstract | ||
| This study includes a physio-chemical and spectral characterization for a biological active compounds in the ANAB Al-Theab plant (Solanum nigrum L.). Three different extracts from dry leaves and fruits (a,b and c) were used to realize this purpose by using different techniques (pre–TLC, m. p., UV–Visible and IR). The obtained results indicated that a major content of those extracts was total crude alkaloid. Whereas, when using (prep–TLC) technique, the value of the Rf of the dispersed spots of extracts was 0.83–0.93 which give positive test with Dragendroff reagent, and the measurements of the melting point (m. p.) of the extracts were 200, 210 and 279°C that closely related to the alkaloid compound ranges. But when Uv–Visible spectroscopy was applied, the value of λ –max was 1.410–2175 measured by spectrophotometer in a wavelength 227.6– 232.0nm. It is known that the absorption of the alkaloid compounds was within the 200–245nm wavelengths. Finally, the data obtained from th IR spectroscopy of the samples, were determined between 600–1360cm which includes the absorption of OH, CH, CO and CN groups, considering as the finger print. These results confirm being the Solanum nigrum plants as indicator for the alkaloids like substances. | ||
| Keywords | ||
| Solanum nigrum; crude alkaloid extracts; Chromatography techneque | ||
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