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PURIFICATION AND CHARACTERIZATION OF Β-GLUCANASE FROM GERMINATED BARELY | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 3, 2010, Pages 299-309 | ||
| Abstract | ||
| β-Glucanase was extracted and partially purified from Iraqi germinated barely(Samir) after sprouting period(3day at 25ºC). The activity was measured by two methods; one for enzyme activity(U/ml) by measuring reducing sugar and second method was relative viscosity for decreasing the viscosity of barely flour solution. The fold of purification and recovery were 6 and 57.14% respectively after the gel filtration by sephadex G-100, this value is about 6 times higher than that of the crud enzyme preparation. The highest activity for the glucanase was at pH 5-6. Whereas the maximum activity was achieved at 60ºC. Results showed that increasing in addition concentration of partially purified β-glucanase caused decreasing in viscosity of barely solution. | ||
| Keywords | ||
| glucanase; germinated barley; glucan; arabinoxylane | ||
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