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STUDYING THE HISTOPATHOLOGICAL AND PHYSIOLOGICAL EFFECTS OF CRUD AND PARTIALLY PURIFIED LIPOPOLYSACCARID FROM Brucella abortus (BIO VAR6) ON LAB MICE | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 3, 2010, Pages 321-336 | ||
| Abstract | ||
| Two hundred fifty blood samples from patients suspected with Brucellosis were collected depending on Rose- Bengal and 2-mercaptoethanol tests. Washing blood samples with distilled water and cultruring on Castaneda-bi phasic medium were used for primary isolation while different morphological, biochmemical beside serological and biotyping tests were performed for final diagnosis. The results showed that 15 isolates were obtained as 9 and 6 isolates for Brucella abortus and Brucella melitensis respecitively. Crude lipopolysaccarid (cLPS) was extracted by hot phenol final concentration 90% from the isolate Brucella abortus (bio var6) then it was partially purified (ppLpS) using Sepharose 4B gel. The quantity of carbohydrate, protein and lipid were estimated for both them. Results showed that the concentrations reached 50, 40, 40µg/ml respectively for cLPS while they were 80, 20, 60 µg/ml respectively for ppLPS. Histopathological and physiological effect of cLPS and ppLPS were studied through detecting the toxic effect and anaphylactic shock on mice. Injection of 0.2ml of cLPS in mice peritoneum caused death within 24 hrs, while injection 0.5ml of ppLPS caused anaphylactic shock and mice death during 48 hrs, from other side injectin 0.25ml ppLPS caused increasing in spleen weight as compared with the control group (which were injected with distilled water). Histopathological section taken from (liver, spleen, kidney, lung) of mice injected with 0.2ml cLPS showed histopathological changes including sever congestion in liver, enlargement in whit pulp, congestion and hemorrhage in spleen, destruction of glomerulars, enlargement in alveolar migration of macrophages while injection of 0.5ml ppLPS caused sever congestion in liver and spleen sinuses and occulation in glomerulars, enlargement in alveolar and slight migration in macrophage in the lung. | ||
| Keywords | ||
| Brucella abortus; Lipopolysaccarid; Histopathlogical and physiological effects on mice | ||
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