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EXTRACTION AND PURIFICATION OF ALKALOID FROM Zephyranthes Candida | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 3, 2010, Pages 360-374 | ||
| Abstract | ||
| This study aimed to extraction and partial purification of narciclasine alkaloid from Zephyranthes candida planting in Iraq. To have semi purified narciclasine alkaloid by using several techniques which includes chromatographic techniques, Colum chromatographic(CL), Gel filteration chromatographic by using Sephadex LH-20 and thin layer chromatographic (TLC). The percentage of alkaloid obtained from this method was 0.25℅.Detection of narciclasine were one as follows: measuring the absorbance by using spectrophotometer at 306 nm, and the retadartion factor (RF) was 0.16 and the melting point was 270-275oC. Results showed that the alkaloid detected might be narciclasine. | ||
| Keywords | ||
| Narciclasine alkaloid; Zephyranthes candida; Purification | ||
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