FFECT OF DICLOFOP - METHYL ON CYTOGENETICS OF HUMAN PERIPHERAL LYMPHOCYTES

	FFECT OF DICLOFOP - METHYL ON CYTOGENETICS OF HUMAN PERIPHERAL LYMPHOCYTES
Iraqi Journal of Biotechnology
Article 1, Volume 9, Issue 3, 2010, Pages 375-384
Abstract
The effect of the herbicide Diclofop –Methyl on Cytogenetic of Peripheral Lymphocytes of Human was studied. Genotoxic effect was studied by recording the chromosomal aberrations (CAs) and induction of Micronuclei (MN), Cytotoxicity recorded by estimating the mitotic index (MI) of cultured cells. The pesticide used at concentrations ( 0.1, 0.5, 5, 25, 50 ) × 10 -5M. Results showed that the pesticide induced an increment in CAs in dose–response pattern. The differences was significant (P<0.01) compared to control treatment and there was significant differences among the concentrations (P<0.01). Among the CAs, broken chromosomes were recorded in control treatment (1±0.06) and a centric chromosomes(1±0.06), such abnormalities appeared upon using lowest concentration (0.1× 10 -5M) without significant difference from control treatment, but in addition ring chromosomes appeared at this concentration. The majority of CAs when using other concentrations were broken chromosomes and reached it's maximum value at concentration 50×10-5 M (10%), ring chromosomes appeared in all treatments. Other abnormalities which has been investigated such as chromosome deletion, translocations, and gene amplification were not recorded in this study. Using the pesticide induced Mn in dose – response pattern with positive correlation coefficient ( r = + 0.97 ). Different concentrations did not effect the MI strongly, although it caused some decreasing with significant difference (P<0.01); the highest concentration 50×10 -5 M raised the MI to 2.67 compared to control treatment ( 1.6) with significant difference (P<0.01) from control treatment and other concentrations.
Keywords
Diclofop; Lymphocytes; cytogenetics; Chromosomal aberration

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