- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
EXTRACTION AND PURIFICATION OF EXTRACELLULAR GLUCOSE ISOMERASE ENZYME FROM ALKALOPHILIC Bacillus sp. BC4 LOCAL ISOLATE | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 3, 2010, Pages 438-446 | ||
| Abstract | ||
| Alkalophilic Bacillus sp.BC4 local isolate, that was isolated from soil, was used to produce extracellular glucose isomerase enzyme, using liquid medium at initial pH of 10 supported with(10ml/l)of xylose extract from straw (H2SO4 straw hydrolysate).The Glucose isomerase enzyme was extracted by centrifugation and purified by two purification steps including fractionation with different saturation of ammonium sulfate(50, 60, 70, 80, 90%) and DEAE-Sepharose CL-6B ion exchange chromatography column. The results showed that 80% saturation of ammonium sulfate gave the best activity for enzyme, where the specific activity reached to 2.61unit/mg protein and overall purification folds to 8.7 with a 78.3% recovery, while the ion exchange chromatography step using DEAE-Sepharose CL-6B column was not observed any enzymatic activity through the wash step, which indicate that most of the enzyme activity is present in the eluted parts and that the enzyme carry a negative charge under the used conditions. The effective eluted parts was collect and calculate each of specific activity was 6.2 units/mg protein and the overall purification folds were 20.6 times with a 47.5% recovery. | ||
| Keywords | ||
| Cellulosic Residues; Glucose Isomerase; Bacillus sp | ||
|
Statistics Article View: 91 PDF Download: 51 |
||