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EVALUATION EFFICIENCY OF NEMACUR PESTICIDES DEGRADABLE LOCAL BACTERIAL ISOLATES IN GREEN HOUSES ENVIRONMENT | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 3, 2010, Pages 518-533 | ||
| Abstract | ||
| Thirty seven isolates of pesticide utilizing bacteria were obtained from green houses soil (previously treated with pesticide) in Baghdad governorate. Most isolates(17) were belonged to the genus Pseudomonas, whereas the others representing the following genera: Bacillus 10 isolates, Streptomyces 7 isolates and Staphylococcus 3 isolates. Streptomyces isolates were selected for the present study. Nemacur utilization was studied using HPLC technique. The results showed that Streptomyces AN1 and AN9 have the highest ability to utilize Nemacur pesticide, since the pesticide completely disappeared from the growing media. Strep. AN1 gave 7 metabolites, one of them was predominant with a peak area of 78.89% and retention time 4.8 and gave the highest dry weight of the biomass 6.1mg/L. Streptomyces AN9 gave 7 metabolites; two of them were predominant with peak area of 68.48% and 17%, and retention time 4.8 and 3.6 respectively. The dry weight of the biomass was 5.0 mg/L. The rest of Streptomyces isolates AN23, AN19, AN30, AN17 and AN8 showed different ability in utilizing Nemacure. The percentages of utilization were 11.4%,6.69%,41.67%,53.18 and 46.74% respectively. These isolates gave biomass of 3.2,4.4, 4.0,4.1 and 4.2 mg/L dry weight respectively. | ||
| Keywords | ||
| Nemacur; bacteria; Degradation; Green houses | ||
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