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STUDY OF PLASMID AND PROTEIN DISCIPLINE OF ESCHERICHIA COLI THAT CONTAMINATION OF MAJOR RIVERS IN IRAQ | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 3, 2010, Pages 548-563 | ||
| Abstract | ||
| Genetic study for ten isolates of Escherichia coli were obtained from highly contaminated water locations belong to the major rivers in Iraq (Mousl, Tikrit, Saad Sammara, Bab Al-Moadham, Diala bridge, Al-Azizia, Al-Kut, Al-Imara, Al-Nasiria and Shate Al-Arab). The isolation of plasmids DNA for the 10 isolates that had multiple antibiotic resistance showed the presence of large and small plasmids bands, also the curing process had done to evaluate the plasmids content for the isolated bacteria by using physical approach (heat) it was found entire losing for the small plasmids, while the large plasmids remain intact. The detection for the protein bands with molecular weight between 14400-94000 Dalton clearly appears in 10% of SDS-polyacrylamide gel that belong to β–lactamase, protease and DNAase, which were the virulene factors for the bacteria. | ||
| Keywords | ||
| coil; Water contamination; Plasmids; Antibiotic resistance | ||
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