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EXTRACTION AND PURIFICATION OF IMMUNOGLOBULIN –G FROM HUMAN BLOOD SERUM | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 3, 2010, Pages 578-591 | ||
| Abstract | ||
| Immunoglobulin G has been extracted from human blood serum by using ammonium sulphate salt in saturation percentage ranged between 0-35%, then immunoglobulin were separated by using ion exchange chromatography with the use of DEAE-Cellulose exchange and to complete separation and purification, gel filtration chromatography technique has been used with Sephadex G-200 column for purification of IgG. The molecular weights of the extracted immunoglobulins was identified with the use of Sephacryl S-200 column, and it was found that the molecular weight of IgG which was extracted from human blood serum was found to be 152 KD. The extracted immunoglobulins concentration was also determined with Bradford method were the concentration was about 9.5% mg/ml. The radial immunodiffusion test was done during all the stages of extraction, separation and purification to insurance degree of purity of immunoglobulins after every stage. | ||
| Keywords | ||
| extraction; Purification; immunoglobulin; human | ||
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