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DIAGNOSIS OF LEPTOSPIROSIS IN HUMAN SERUM AND URINE SAMPLES USING PCR TECHNIQUE | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 9, Issue 3, 2010, Pages 630-641 | ||
| Abstract | ||
| This study was focused on the using of PCR technique for diagnosis Leptospirosis as the the diagnosis of Leptospirosis using traditional methods is difficult because Leptospira is considered as fastidious bacteria. Therefore, Polymerase Chain Reaction (PCR) technique was used to diagnose leptospirosis using serum and urine of human. Samples were collected from human suspected with Leptospirosis (50 urine and 50 serum. DNA was extracted using a special kit, DNA purity and integrity was conducted. PCR was performed using specific primers.The product was electrophorised on agarose gel and detected using ultraviolet transillumination with DNA marker in addition to standard DNA for leptospira. The result was revealed that through the examination of the 100 samples, 7 cases of human urine samples were positive while human serum samples were negative results. The results have also revealed some positive cases in PCR of those gave negative results in extraction of DNA. This study proved that the validity of PCR technique for diagnosis of Leptospirosis. This technique was sensitive, accurate and achieved in short time and the possibility of using urine and serum of human for the diagnosis of the infected cases. | ||
| Keywords | ||
| Leptospira diagnosis; PCR; Specific primers | ||
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