- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
ENUCLEATION... THE STARTING POINT FOR BIOLOGICAL CLONING | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 10, Issue 2, 2011, Pages 149-167 | ||
| Abstract | ||
| The Enucleation Technique considered as the first and most important step to accomplishe any biological cloning process which includes also Fusion; Activation ,and Embryo transfer. This Review Article was carried out to elucidate, the different Enucleation methods since the first attempt of “Brigg”and “King” (1952) and by others till (2011) including the physical methods such as UV Irradiation to Enucleate the Amphibian nuclei or using Microsurgery and/or both Techniques. The Microsurgery technique includes two methods: the Enucleation of nuclei of the MII Oocyte and the Enucleation of nuclei of the Telophase II Oocyte. The review article includes also the Enucleation using the chemical methods which includes the cytochalsin B; Enucleation using Hypertonic solution; and Enucleation using Demecolcine and Nocadazole. | ||
| Keywords | ||
| Enucleation; Microsurgery; Telophase II; Hypertonic; Demecolcine Nocadazole | ||
|
Statistics Article View: 38 PDF Download: 12 |
||