THE EFFECT OF SODIUM FLUORIDE ON HISTOLOGICAL CHANGES

	THE EFFECT OF SODIUM FLUORIDE ON HISTOLOGICAL CHANGES
Iraqi Journal of Biotechnology
Article 1, Volume 7, Issue 2, 2008, Pages 147-156
Abstract
The present study was conducted to identify the effect of oral administration of sodium fluoride (NaF) on histological changes of ovarian and uterine horns in mice. Sixty four mature female Swiss albino mice, Balb/C were divided into four major groups including control (C) group and treatment groups (T1 , T2 and T3) according to NaF dose as following 0 ppm, 200 ppm, 400 ppm and 600 ppm , respectively. Each major group was subdivided into two minor groups according to the period of administration in 10 and 15 weeks. Therefore, each minor group contains eight females. Histological changes were assessed includes ovarian diameter, number and diameter of growing ovarian follicle (GOF), Graafian follicles (GF) and corpus luteum (CL), diameter of uterine horn (UH), thickness of epithelial cell layer (ECL) lining the UH and diameter of uterine glands (UG). The results of ovarian histological changes showed a highly significant (P<0.01) reduction in the ovarian diameter, number and diameter of GOF, GF and CL for all treated groups T1, T2 and T3 as compared to control for both treatment periods (10 and 15 weeks). Within same treatment periods, histological examination of uterine horn observed highly significant (P<0.01) reduction in the diameter of UH, thickness of ECL and UG for all treated groups as compared to control. It was concluded that the administration of NaF to female mice have harmful effects on histological changes of ovaries and uterine horns; and consequently may have a negative effects on female fertility and fecundity.
Keywords
Sodium fluoride; female mice; Fertility

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