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GROWTH OF STAPHYLOCOCCUS AUREUS AND PRODUCTION OF HEMOLYSIN IN DIFFERENT PLANT AND ANIMAL WASTES MEDIA | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 7, Issue 2, 2008, Pages 157-167 | ||
| Abstract | ||
| Four different media prepared from plant and animal wastes included rice bran, sunflower meal, potato peels and chicken bones, further more Brain Heart Infusion was used as commercial medium. The media was prepared as solid media (with agar and blood) and liquid media in two concentrations 5% and 10%.The media were cultured with S. aureus, growth and hemolysin were detected in solid media and quantitatively estimated in liquid media. S. aureus grew and produced hemolysin in all these media. The highest growth was observed in 5% and 10% rice bran. Hemolysin activity in 5% rice bran was more than in other media, followed by sunflower meal, ckicken bones and potato peels respectively. The bacterial growth in 10% concentration was more than in 5% in most media, while hemolysin activity was nearly equal in the two concentrations, except in rice bran in which hemolysin in 10% was more than in 5%. These results indicated that the plant and animal wastes used are suitable for bacterial growth and hemolysin production, rice bran is the best one for this purpose. | ||
| Keywords | ||
| Staphelococcus aureus; Growth; hemolysin; waste media | ||
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