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Comparison betweenmethods Of preparation For Gold Nanoparticles with Glutathione | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 25, Issue 1, 2014, Pages 45-50 | ||
| Author | ||
| Ikteffa M. Al-Hasnawi | ||
| Abstract | ||
| In this study, we were conductedcomparison between twomethods ofpreparationcoatedglutathione(GSH) withgold nanoparticles(GNPs). In first we were attendedthedirectGNPswithGSH , theresults of the absorbance characteristics of this method were monitored using UV-Vis spectrophotometer ( at 526 nm ) and the corresponding AFM image for the as-prepared GSH-GNPs were dispersed with about 10 nm in diameter.In second method we were attended GNPs that have UV-Vis spectrophotometer ( at 527 nm ) and then using GSH as capping agent for protective layer , UV-Vis spectrophotometer ( at 545 nm ) , and the corresponding AFM image for the capped GSH-GNPs were dispersed with about 20 nm in diameter. The change in the color of the colloid was also seen before and after capping. The color of the gold colloid changed from yellow to red in first method,but changed wine red to blue for glutathione capped nanoparticles in second method ,in which glutathione capped gold nanoparticles were prepared by borohydridereduction,which were directly used for covalently attaching an enzyme .peptide or protein and the resulting AuNP- bioconjugate. | ||
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