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Molecular Identification of Entamoeba histolytica Parasite by Using Actin and Amebapore-A Genes | ||
| kufa Journal for Nursing sciences | ||
| Article 1, Volume 4, Issue 2, 2014, Pages 48-55 | ||
| Author | ||
| Jameel Jerri Yousif Al-Hameedawi | ||
| Abstract | ||
| Objective: The present study was conducted in the province of Al- Najaf, for the period from April 2013 until November 2013 , which aims to identify the Entamoeba histolytica parasites by screening for Actin and Amebapore – A genes in stool samples by using the real-time PCR technique. Methodology: The study included , collect ( 104 ) stool samples from patients with diarrhea arrivals to Al-Sadar Medical City Hospital ,Al- Zahra Maternity and Children Hospital , Al-Manathera hospital and Al-Sajjad Hospital. Results: The results of the real-time PCR revealed , the presence of E. histolytica parasites in ( (25) stool samples out of the ( 104 ) samples, the rate ( 24%) . Conclusion: In light of the results obtained through this study we concludes that the efficiency of real-time -PCR technique large and rapid in diagnosis of E. histolytica parasites in stool samples. Recommendation: The study of gene expression of Actin and Amebapore – A genes, specific for E. histolytica parasite. | ||
| Keywords | ||
| Real; time PCR; Entamoeba histolytica; Actin; Amebapore | ||
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