Study Of Extended-Spectrum ß-lactamases Producing Enterobacteria isolated from Cockroaches ( Periplaneta americana ) of Central Medicine City Hospital

Faraj Al- Marjani, Mohammed

	Study Of Extended-Spectrum ß-lactamases Producing Enterobacteria isolated from Cockroaches ( Periplaneta americana ) of Central Medicine City Hospital
Al-Mustansiriyah Journal of Science
Article 1, Volume 19, Issue 1, 2008, Pages 9-16
Author
Mohammed Faraj Al- Marjani
Abstract
Fifteen American Cockroaches ( Periplaneta americana ) were captured to determine the potential role of the cockroaches in carrying Extended-Spectrum ß-lactamases producing pathogens from Central Medicine City hospital. Only cockroaches captured whole and live were utilized for the study. After that each cockroach was placed in a test tube with 5 ml sterile saline solution , and then homogenized. The resulting solution was cultured on the culture media. 32 isolates of Enterobacteriaceae were identified , the most frequent were Klebsiella pneumoniae (25%), Shigella spp.(18.7%), Enterobacter aerogenes and Proteus mirabilis( each 15.6%) , Serratia marcescens (12.5%) and E. coli ( 3.12%) . Bacterial isolates were tested against (11) antimicrobial agents: Amoxicillin, Cefotaxime ,Imipenem, Ciprofloxacine, Aztreoname , Cefuroxime , Nalidixic acid , Trimethoprim , Tetracycline , Mezlocillin and Gentamicin . Results showed that all the isolates were resistant to Cefuroxime , Amoxycillin , and all the isolates have showen multiple resistance for antibiotics. All isolates (100%) were susceptible to imipenem. The majority of isolates remained susceptible to Ciprofloxacine (78.2%) and Aztreoname (68.8%) . The results showed also that 18 isolates ( 56.25 %) had the ability to produce ß -lactamase enzymes , 5 isolates ( 15.6% ) were able to produce Extended-Spectrum ß -lactamases ( ESBLs) . DNA analysis ( Plasmid profile) showed that 59.37 % of the Bacterial isolates contained plasmid of different molecular weights.

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