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Purification of a Restriction Enzyme from Aspergillus niger and Some General Properties | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 19, Issue 2, 2008, Pages 27-37 | ||
| Author | ||
| Mukaram Shikara | ||
| Abstract | ||
| A restriction enzyme has been purified from 7-day Aspergillus niger culture by a procedure included the recovery of the enzyme by ammonium sulphate, DEAE-cellulose and Sephadex G-100. The purification procedures were complicated with the presence of carbohydrates and polyphenolic pigments. The enzyme has been tested against lambda DNA and comparison with other standard restriction enzymes. The enzyme has been stable with 419-fold purification with the recovery of 40%. | ||
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