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Optimum Conditions of Protease Production from Bacillus licheniformis (B1) and its Applications | ||
| JURNAL OF MADENAT ALELEM COLLEGE | ||
| Article 1, Volume 6, Issue 1, 2014, Pages 26-39 | ||
| Authors | ||
| Haider Jawad Kadhim; Sanaa Burhan Aldeen | ||
| Abstract | ||
| Forty isolates of Bacillus spp. were isolated from sixty samples including; soil, water and meat. Ability of these isolates to produce protease was evaluated. Bacillus B1 isolated that isolated from soil showed the highest protease production. it was identified as a strain of Bacillus licheniformis.The optimum culture medium and conditions for protease production were casein-yeast extract medium contained soluble casein (0.5g), yeast extract (0.5g), glucose (1g), KH2PO4 (0.02g), K2HPO4 (0.02g) and MgSO4.7H2O (0.01g), in 100ml distilled water, pH 8.0 and incubated at 37˚C. for 48 h. The crude protease exhibited ability to remove the blood color from cloth within 30 min and gelatin from X-ray film within 120 min. | ||
| Keywords | ||
| Protease; Production; Bacillus licheniformis; applications; Bacillus licheniformis B | ||
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