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Total Antioxidant Status and Genetic Polymorphism of SOD2 and SOD3 genes Associated to Diabetic patients in Major β- Thalassemia | ||
| Journal of University of Babylon | ||
| Article 1, Volume 22, Issue 6, 2014, Pages 1754-1767 | ||
| Author | ||
| Moaed E. Al-Gazal ly | ||
| Abstract | ||
| Oxidative stress can be induced in different ways, some of which are connected with known pathologies. Deficiencies in antioxidant or related enzymes are the factors which can result in oxidative stress. Genetic polymorphism is the most common cause of these deficiencies. There were statistically a highly significant elevated (p<0.001) in total antioxidant status(TAS) , Bilirubin(Bil.) and uric acid(UA) in β- thalassemia major with diabetic patients(No.51), when compared with β- thalassemia major(No.55) and control groups (No.50). The results showed a highly significant increase (p<0.001) of Mn-SOD (SOD2) and EC-SOD(SOD3) activities in β-thalassemia major with diabetic patients when compared with β-thalassemia major patients and control groups. The present study was performed assess whether there is a genetic association between a functional polymorphism (Ala-9Val) in the human Mn-SOD (SOD2) and EC-SOD(SOD3) genes in major β- thalassemia, major β- thalassemia with diabetic and control using PCR/RFLP method. The results shows frequencies of the Ala allele (64.1% vs. 51.1%, p < 0.001) and the Ala/Ala genotype (29.39% vs. 23.51%, p < 0.001) of the SOD2 gene were significantly lower in major β- thalassemia with diabetic patients than in major β- thalassemia. In contrast, the Val allele (35.9% vs. 48.9%, p < 0.001) of the SOD2 gene were significantly increased in major β- thalassemia with diabetic patients than in major β- thalassemia and the Val/Val genotype (13.76% vs. 13.76%, p < 0.01) were no significantly between groups. Significance difference in the frequencies appears in Arg213GlySOD3 ,Arg/Arg, Gly/Gly genotype between major β- thalassemia with diabetic and major β- thalassemia. The present study provides some evidence that genetic polymorphism in the SOD2 and SOD3 genes associated with increased risk of major β- thalassemia with diabetic among major β- thalassemia with high levels of oxidative stress .This suggests that the Ala(-9)Val dimorphism in the SOD2 gene is associated with major β- thalassemia with diabetic. | ||
| Keywords | ||
| Total antioxidant; major; thalassemia; Diabetic; genetic polymorphismSOD; polymorphismSOD | ||
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