In-vitro Comparative Study of Antibacterial activity of Syzygium aromaticum with three Antibiotics Against E. coli

M. Hady, Oday; Saleem Raheem, Saad; Abbas Hasan, Ahmed

	In-vitro Comparative Study of Antibacterial activity of Syzygium aromaticum with three Antibiotics Against E. coli
Kufa Journal For Veterinary Medical Sciences
Article 1, Volume 5, Issue 1, 2014, Pages 73-84
Authors
Oday M. hady; Saad Saleem Raheem; Ahmed Abbas hasan
Abstract
The results confirmed antibacterial activity with significant differences (P≤0.05) of clove (Syzygium aromaticum) in the concentration 10µg/ml(MIC) (1.10+ 0.31). Also superiority of the antibiotic (Doxycycline) that has MIC (Minimum Inhibitory Concentration) at 10 µg/ml by significant differences (p≤0.05) (2.00+0.08) aginst E . coli that appears sensitive to its, while it doesn’t clear any significant differences in both of the antibiotics (Gentamicin and Gentadoxycycline). In case antibacterial activity of antibiotic (Gentamicin) against E . coli by sensitive tests observe resistance of E . coli , started from 10 µg/ml (1.60+0.15) to 100 µg/ml (2.30+0.05) by significant differences (p≤0.05). Also the antibiotic (Gentadoxycycline) by combination of (Gentamicin and Doxycycline) in-vitro ,it doesn’t show any significant differences (p≤0.05) when applicated the sensitive tests of the antibiotic against E . coli at concentration 10 µg/ml (1.80+ 0.15) and 100 µg/ml (2.60+ 0.19), which demonstrates antagonist between them; by numbering of inhibition zone appeard that the results less than (Doxycycline) and more than (Gentamicin), As a whole, these results illustrate superiority of Doxycycline as a antibacterial activity against E . coli depended on sensitivity tests according to antibiotics(Gentamicin and Gentadoxycycline) and clove(Syzygium aromaticum) by significant differences (p≤0.05) . Objective Knowing of antibacterial activity of clove (Syzygium aromaticum) against bacteria (Escherichia coli ) in in-vitro, and compare of it with three antibiotics (Gentamicin, doxycycline and Gentadoxycycline), by measurement of inhibition zone for both of them. Material and Methods Bacteria (E . coli ) was isolated from the patients in the city of al- Sadir medical on E.M.B (Eosin Methylene Blue) agar and incubated at 37 o C for 18-24 hours. This trial is performed in in-vitro (sensitivity test) by agar well diffusion technique, in diameter of well (8 mm) and by measurement of inhibition zone in nutrient agar after 24 hrs, and knowing of Minimum Inhibitory Concentration (MIC) for both of them. Conclusions Syzygium aromaticum has high antibacterial activity against the Bacterial growth of (Escherichia coli). Doxycycline has high antibacterial activity against the Bacterial growth of (Escherichia coli). Resistance of E.coli to the commonly used eradication antibiotic drug(Gentamicin). The combination between two antibiotics (Gentamicin and doxycycline ) doesn’t give antibacterial activity against the Bacterial growth of (Escherichia coli).
Keywords
Clove; Syzygium aromaticum; antibiotic; Gentamicin; doxycycline; Gentadoxycycline

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