Comparative histological study between the hepatoprotective effect of Quercetin and Trigonella fenum graecum extract in carbon tetrachloride induced hepatotoxicity at male rats

Hassoon Al-Qaraghuli, Noor; Mahmood Al-Jammali, Saher; Dakhil Al-Rekabi, Mohammed

	Comparative histological study between the hepatoprotective effect of Quercetin and Trigonella fenum graecum extract in carbon tetrachloride induced hepatotoxicity at male rats
Kufa Journal For Veterinary Medical Sciences
Article 1, Volume 5, Issue 1, 2014, Pages 43-54
Authors
Noor Hassoon Al-Qaraghuli; Saher Mahmood Al-Jammali; Mohammed Dakhil Al-Rekabi
Abstract
The liver is the main organ which responsible for the removing of toxins in the body, making it the first in the face of various chemicals from the digestive system, and according to this the study had designed to compare the hepatoprotective effect of the Quercetin and Trigonella foenum graecum extract on the liver tissues of male rats were poisoned with carbon tetrachloride, and to know their effects when using together. The study was conducted at the Faculty of Pharmacy - University of Kufa, and included the using of 35 rats from Sprague-Dawley divided into five groups: the control group, the positive control group, the group treated with Trigonella foenum graecum extract, the quercetin treated group and the group treated with Trigonella foenum graecum extract and quercetin together. The period of dosing was continued for 7 days and on the eighth day all groups(except the control group) were poisoned with carbon tetrachloride. After 24 hours the animals were killed. The study lasted from April 2013 - until July 2013. The Glutathione and Malondialdehyde levels were estimated in liver homogenized. The results were showed the following: no significant difference (P>0.05) in the Glutathione level and a significant increment (P<0.05) in the level of Malondialdehyde at the treated groups when compared with the control group. Also the results were obtained a significant decrease and increase (P<0.05) in the levels of Glutathione and the Malondialdehyde at the treated animals as compared with the positive control group. The present study was showed numerous histological changes were resulted from the injection with carbon tetrachloride. In related with the tissue sections which have been taken from the treated groups: with the extract, quercetin and the group treated with the together were revealed a protective action for the seeds of Trigonella foenum-graecum and quercetin drug on the liver tissues which had been poisoned and the best effect in the protection was showed at the group which treated with the extract and quercetin together. From this study we can conclude that the Trigonella foenum-graecum extract and quercetin may reveal a protective role for the liver against carbon tetrachloride poisoning by increasing the Glutathione level and fall - off the Malondialdehyde level in liver homogenized. Also we conclude the using of Trigonella foenum-graecum extract and quercetin together led to a high efficiency double protection for the liver tissues as a result of the positive impact which resulted from their using together.

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