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Detection of the genetic stability for the tissue tomato plants by using RAPD technique | ||
| journal of kerbala university | ||
| Article 1, Volume 7, Issue 3, 2011, Pages 208-217 PDF (0 K) | ||
| Abstract | ||
| The Randomly Amplified Polymorphic DNA (RAPD) markers were used to confirm the genetic stability for two hybrids of tomato: Shorouk and GS-12 produced from plant tissue culture which produced from synthetic seeds were selected and randomly assigned to three treatments as well as the control treatment which included plantlets developing from the tomato seeds planted on the sterile filter paper and moisturized with sterile distilled water. RAPD-PCR analysis using 5 universal primers were performed on DNA extraction from the fresh healthy leaves of the mother plants and from samples randomly taken plantlets derived from tissue culture for both tomato hybrids. Results have been obtained by using the primer OPC-O3 which elect out of five primers confirmed the resemblance among the tissue culture seedlings which grown from synseeds (synthetic seeds) as well as with those grown from natural seeds, furthermore RAPD appears to be an efficient technique and a simple fast DNA marker for the early detection of genetic variation in plants produced by tissue culture technique. | ||
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