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EFFECT OF SUCROS AND PHENYLALANIN ON ACCUMALATION OF TROPANE ALKALOIDS IN BALLADONNA Atropa belladonna CALLUS IN VITRO | ||
| journal of kerbala university | ||
| Article 1, Volume 7, Issue 3, 2011, Pages 280-292 PDF (0 K) | ||
| Abstract | ||
| An experiment was conducted at tissue culture lab .,Dept of Hort., College of Agriculture , University of Baghdad . during 2009-2010 . The study included effect of each sucrose and phenylalanine on fresh and dry weight of callus derived from shoot tip of belladonna and on accumulation of tropane alkaloid in callus which included (Atropine ,hyoscyamine , hyosine or scopolamine) . Results indicated the treatment 30 g.l-1 sucrose with 20 mg.l-1 phenylalanine gave the highest fresh and dry weight of callus (495.7 -52.4) mg respectively , while the treatment 60g.l-1 sucrose with 10mg.l-1 phenylalanine gave the highest concentration of Atropine and Hyoscyamine of (528.73 -70.29) µg.Gdw-1 respectively , while the treatment 60g.l-1 sucrose with 40mg.l-1 phenylalanine gave the lowest concentration of Atropine of 65.90 µg.Gdw-1 . | ||
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