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Effect of Amino Acids Type and Concentration on Callus Induction from Mature Bean Phaseolus vulgaris L. Embryos | ||
| journal of kerbala university | ||
| Article 1, Volume 7, Issue 3, 2011, Pages 612-619 PDF (0 K) | ||
| Abstract | ||
| This research was conducted to study the effect of some amino acids and their concentrations on callus induction from mature bean CV. Harvester embryos. Calli were induced on MS media supplemented with 0.5 mg /L BA, 1 mg/L IAA and different combination and concentrations of casein hydrolysate, glycine, asparagine, tyrosine and myo inositol as described in the text. All the embryo parts induced calli on the control medium which contained 100 mg/l of each of casein hydrolysate, glycine, asparagine, tyrosine, and myo- inositol, with different percentage. The hypocotyl apex gave significantly high percentage (95.6%) of callus induction compared with the radicle (76.7%) and the shoot apex (36.6%). | ||
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