Effect of fungi inoculation on alkaline phosphatase enzyme activity in sterilized soil

Faisal Said, Mazin; Ayad Harbawee, Mohammed

	Effect of fungi inoculation on alkaline phosphatase enzyme activity in sterilized soil
Journal of Tikrit University For Agriculture Sciences
Article 1, Volume 14, Issue 0, 2014, Pages 142-148
Authors
Mazin Faisal Said; Mohammed Ayad Harbawee
Abstract
Factorial experiment was conducted in laboratory using complete randomize designe (CRD) to study the effect of interaction between inoculation with phosphorus solubilizing fungi, Aspergillus niger , Tricoderma spp. and Penicillium spp. which isolated from forest soil and organic residues (sheep manures) on alkaline phosphatase enzyme activity and fungi numbers in sterilized clay soil under different incubation times 10 , 20 , 30 and 40 days. The results revealed that inoculation with A. niger gave significant increase in alkaline phosphatase enzyme activity equal to 261.98 μg Para Nitrophinol Phosphate "PNP". mg-1. Soil h-1 as compared with respectively , T. spp. and P. spp. which gave values equal to 233.16 and 219.62 μg Para Nitrophinol Phosphate "PNP". mg-1. Soil h-1 respectively. Treatment amended with sheep manures gave significant increases in enzyme activity of 8.94% as compared with unamended treatment. 30 days incubation gave the highest value for enzyme activity equal to (345.39 μg PNP. mg-1. Soil h-1). Treatment inoculated with A. niger , amended with 2% sheep manures and incubated for 30 days gave the bast values for enzyme activity equal to (419.63 μg PNP. mg-1. Soil h-1)which increase significantly as compared with all other treatments. Fungi number was increased significantly by increase incubation time till to 30 days. Treatment inoculated with A. niger, amended with 2% sheep manures and incubated for 30 days gave the highest fungi number equal to (7.3 × 105 Colony Formation Unite "CFU". gm-1 dry soil).
Keywords
Fungi; soil

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