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Effects of soluble ß-glucan on the immune responses of broiler chickens vaccinated with Newcastle disease vaccine and reared under heat stress | ||
| The Iraqi Journal of Veterinary Medicine (IJVM) | ||
| Article 11, Volume 38, Issue 1, 2014, Pages 30-39 PDF (0 K) | ||
| Authors | ||
| Ihab Ahmed Naser; Nidhal Raoof Mahdi | ||
| Abstract | ||
| The main objectives of this study was to determine the influence of soluble β-glucan extracted from the cell wall of Saccharomyces cerevisiae on immune response of broiler chickens reared under heat stress .β-glucan 225μg/ml was supplemented in drinking water to broiler chicken vaccinated with Newcastle disease virus (NDV) vaccine. The parameters of the assessment of the immune response was the Heterophil / Lymphocyte ratio as a measure of stress, determination of the serum antibody titer post vaccination with the NDV vaccine by ELISA test and Immunohistochemical detection of macrophages by using monoclonal antibodies (mouse anti-chicken macrophage KUL01). A hundred and twenty (120) Chickens one day old were divided into two equal groups; group under heat stress and group control; each group was divided into two subgroups (G1, G2, G3 and G4) containing thirty chicks. The experiment was conducted for six weeks. The stressed group exposed to heat stress (≃35ºC) starting from the third week of age upto the end of the experiment. While (Group 1) and (G3) chicks were supplemented with 225μg/ml of soluble β-glucan in drinking water from day 1 to the end of the experiment, while (G2 and G4) chicks were not supplemented. The result of Heterophil /Lymphocyte ratio indicates that there was a significant (P < 0.05) difference within heat stressed treated which showed an elevated H/L ratio at 21,28 and 35 days old. Also there was a significant (P < 0.05) difference between groups that were treated with β-glucan (G1, G3) at 21, 28 and 32 days of age compared with a control non treated non stressed group (G4) at same periods. The results of antibody titer to NDV showed that there were significant (P < 0.05) differences among all groups at 7, 14 and 21 days of age, and the results of immunohistochemical analysis demonstrated positive staining for duodenal and bursal macrophages labeled with KUL-01 mouse anti-chicken monocyte- macrophages monoclonal antibodies. Tissue sections of duodenum and bursa at 14 and 24 days old stressed and non-stressed groups treated with β-glucan showed a positive result (purple-brown staining macrophages) in G1 and G3 as compared with duodenal and bursal tissues of G2 and G4groups which showed no stained cells. | ||
| Keywords | ||
| glucan; Newcastle disease virus vaccine; Immune response; Broiler Chickens | ||
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