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Molecular identification by multiplex polymerase chain reaction of Pasteurella multocida in cattle and buffaloes in Baghdad | ||
| The Iraqi Journal of Veterinary Medicine (IJVM) | ||
| Article 20, Volume 38, Issue 1, 2014, Pages 99-106 PDF (0 K) | ||
| Authors | ||
| A. M. H. Al-Judi; Ihab G. M. AL-Shemmari | ||
| Abstract | ||
| The aim of this study was to identify pasteurella multocida and their types by PCR in cattle’s and buffaloesi bagdad from March to August 2012 on 204 animals , including 102 cattle and 102 buffaloes at slaughter houses from Baghdad .Blood samples and nasal swaps were collected , before slaughtering and lung tissues of slaughtered animal , and from 54 clinically suspected cases of pasteurellosis , including 27 bovines ,and 27 buffaloes the samples taken included blood and nasal swabs . Pasteurellamultocida were isolated from 94 animals include 49 cattle 45 buffaloes. The typing of the isolates by multiplex PCR for genotyping Pasteuerllamultocida revealed 93 isolates of type B , 31 from cattle and 62 from buffaloes ,and 81 isolates of type A , 55 from cattle and 26 from buffaloes . | ||
| Keywords | ||
| Pasteurella multocida; Multiplex PCR; cattle; Buffaloes | ||
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