EFFECT OF SOAKING SEEDS IN β-AMINOBUTYRIC ACID TO INDUCE SYSTEMIC RESISTANCE IN ZUCCHINI PLANT AGAINST ZUCCHINI YELLOW MOSAIC VIRUS

M. Jarjees, M.; R. Merzah, N.

	EFFECT OF SOAKING SEEDS IN β-AMINOBUTYRIC ACID TO INDUCE SYSTEMIC RESISTANCE IN ZUCCHINI PLANT AGAINST ZUCCHINI YELLOW MOSAIC VIRUS
The Iraqi Journal of Agricultural Science
Article 1, Volume 45, Issue 5, 2014, Pages 518-526
Authors
M. M. Jarjees; N. R. Merzah
Abstract
This study was conducted to evaluate the efficiency of β-aminobutyric acid (BABA) in inducing systemic resistance in zucchini plants (c.v amjad) against Zucchini yellow mosaic virus (ZYMV). BABA was used at 1% to soak zucchini seeds for 6 hours. The efficiency of BABA was evaluated on the bases of inhibition percent of virus multiplication as estimated by ELISA value and peroxidase activity. Results revealed that ELISA value of seed soaked in β- aminobuytric acid and directly planted then inoculated with ZYMV and sprayed again with the same acid was 0.248 at 405 nm and the percent of virus multiplication inhibition was 88.61% .The total absorbance change of peroxidase attained to 65.06 and 56.26 after 7 and 21 day respectively. This treatment was significantly different from control treatment in which the seeds soaked in distilled water and planted, then inoculated with ZYMV. ELISA value was 2.179 and peroxidase absorbance 32.73 and 36.93 after 7 and 21 day respectively and the percent of virus multiplication inhibition of 0%. The β- aminobuytric acid was highly efficient effect on reducing losses in number and weight of zucchini fruits.
Keywords
Cucurbita pepo; ELISA; cowpeas; Faba beans; immunostrips

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