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Emergence of plasmid mediated aac(6´)-Ib-cr Gene in Flouroquinolon- resistant Acinetobacter spp. | ||
| Al-Qadisiah Medical Journal | ||
| Article 1, Volume 10, Issue 1, 2014, Pages 96-107 PDF (0 K) | ||
| Authors | ||
| Adnan H. Al-Hamadani; Ali M. Al-Mohana; Ali S. Al-Khazaali | ||
| Abstract | ||
| This study aimed to detection of plasmid mediated aac(6´)-Ib-cr gene in fluoroquinolon resistant Acinetobacter spp. A total of 154 clinical and inanimated samples were collected from inpatients and medical devices from different units in Al-Dewaniyah Teaching Hospital during a period of five months from November 2011 to March 2012. Sources of specimens were ICU (n= 85), burn unit (n=43) and urine from inpatients suspected with UTI (n=26). Totally, 16(10.3) Acinetobacter spp. were obtained. Identification of Acinetobacter isolates to species level was done by VITEK 2 compact system, results showed that the vast majority of isolates belong to A. calcoaceticus A. baumannii complex (15, 93.8%) while only one isolate (6.3%) was belong to species A. lowffii. All of the 16 Acinetobacter spp. isolates were testwd to susceptibility against 20 antibiotics by VITEK 2 system with GN-AST23 cassettes. Result showed that all of isolates were resistant to at least three classes of antibiotics. Isolates showed high resistance rates to first-, second-, third- and fourth generation cephalosporin, aminoglycosides and nitrofurantoin. Results revealed that 11 isolates were fluoroquinolon resistant. DNA of the fluoroquinolon resistant isolates was extracted and used in PCR assay. Results showed that 4(32.3%) isolates were harboring aac(6´)-Ib-cr gene. | ||
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