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Isolation and Characterization of New Pseudomonas aeruginosa Phages in Al- Diwanyia City | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 13, Issue 3, 2013, Pages 10-22 PDF (0 K) | ||
| Authors | ||
| Ziad M.F. Alkhozai; M.F. Alkhozai | ||
| Abstract | ||
| This study was carried out during the period from October/ 2011 to March/ 2012.It included the isolation and identification of 50 Ps.aeruginosa isolates from 200 samples collected from different clinical and environmental sources and detection of their susceptibility to eighteen antibiotics. The distribution of these isolates were: 21 from 50 burn samples, 4 from 40 UTI samples,10 from 50 samples of operation rooms and 15 from 60 sample of sewage water. The obtained results showed that all Ps.aeruginosa isolates were sensitive to imipenem and meropenem. Five types of phages were isolated from (60) samples of sewage water according to difference in shape and diameter of the plaques primarily named (ZM1,ZM2,ZM3,ZM4,ZM5). Results showed that the highest adsorption rate of isolated phage on bacterial cells was about 3-5 minutes, phage particles have an eclipse time about 7.5 ±0.5 minutes. The maximum lysis activity of phages was documented in about two hours, while complete lysis time was about 5 hours. The calculated data showed that the burst size was about 125 ±18 pfucell in time period about 30 ±5 minutes. The results also showed that the Ps.aeruginosa isolates from sewage were more susceptibleto phage infection than isolates from clinical cases. The isolated phage arrived the maximum activity in the neutral medium between 6.5-8.5. This activity declined after pH 9. In 45Cº the phage activity was not affected but it decreased continuously when temperature increased 50Cº. In 70Cº the activity dramatically reduced and diminished in about 8-10 minutes. Scanning Electron Microscope results showed the phage particles possessing icosahederal head and diameter ranging between (51-74nm) with no tail. According to International Committee on Taxonomy of viruses, the phage (ZM1) was assigned to the family Corticoviridae. | ||
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