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Detection of some virulence factors of Enterococcus faecalis isolated from raw milk by Multiplex PCR | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 13, Issue 3, 2013, Pages 132-144 PDF (0 K) | ||
| Author | ||
| Azhar Noory Hussein | ||
| Abstract | ||
| Since milk is a high nutrition food , it is extremely liable to enterococcal contamination , as this widely distributed in the environment . This study evaluated 40 raw milk samples ( 10 samples for each cows , sheep , goats and buffalo ) for enteroccal contamination . Raw milk samples were collected from different farms in Diwaniyah city /Al-Qadisiya governorate between May and July 2012. This objective of this study was numerate the number of enterococci in the raw milk samples . Also to find out the out the distribution of major virulence factors asa1 , gelE , cylA , esp and hyl genes by multiplex PCR among Enterococcus faecalis isolates and the production of gelatinase and hemolytic activity were also determined . The antibiotics susceptibity of the strains was determined using the disc diffusion method . The results of the current study showed that the raw milk had a total viable count in the range from 1*102 – 34*106 CFU/ml and all raw milk samples were positive for the presence of enterococci . Significant difference was not exist between the types of raw milk samples . twenty strains of E. faecalis were isolated from 40 raw milk samples . These strains were identified by conventional biochemical tests and the HiStrep system . Tn addition , E. faecalis specific 16S rDNA gene (amplicon size 290 bp ) primers were included as a control when the biochemical identification was doubtful . Multiplex PCR for virulence factors showed that E. faecalis isolates carried one or more virulence encoding genes . The asa1 gene was the predominant (75%) of the E. faecalis strains investigated followed by hyl gene (70%) . The esp , gelE and cylA genes were detected with percentages of 65% , 50% and 25% respectively . Antibiotics susceptibility testing showed that of the total 20 isolates 12(60%) were resistant to erythromycin and 9(45%) to ampicillin . Lower antibiotics resistance was seen with gentamycin , tetracycline , norfloxacin , penicillin and rifampicin ( 20% , 15% , 10% , 15% and 10% respectively ) . None of the isolates was found to be resistant to be chloramphenicol . | ||
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