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Spectrophotometric Determination of Chloramphenicol in Pharmaceutical Preparations | ||
| Iraqi National Journal Of Chemistry | ||
| Article 1, Volume 0, Issue 55, 2014, Pages 231-242 | ||
| Authors | ||
| Wasan Abdul Amir Al-Uzri; Sadeem Subhi Abed; Mouyed Qassim Al-Abachi | ||
| Abstract | ||
| Abstract Two simple, rapid and sensitive spectrophotometric methods have been developed for the determination of chloramphenicol (CAP) in pure form and in pharmaceutical preparations. The proposed methods are based on a coupling reaction between reduced chloramphenicol (by zinc powder and concentrated hydrochloric acid) with chromatropic acid or phenol in the presence of ammonium hydroxide to form an intense red violet or yellow color, water-soluble dyes that are stable and have a maximum absorption at 515 nm using chromatropic acid and 432 nm on using phenol. The calibration graphs were linear over the concentration ranges of 0.52 -12 µg mL-1 and 0.4- 18µg mL-1 with a limit of detection (LOD) of 0.1334 µg mL-1 and 0.0873 µg mL-1 for chromatropic acid and phenol, respectively. The proposed methods were successfully applied to the determination of CAP in ointment and eye drops. | ||
| Keywords | ||
| Chloramphenicol; chromatropic acid; phenol spectrophotometry; diazotization | ||
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